Exploring the unusual: a testosterone-secreting ovarian tumor

ABSTRACT Ovarian steroid cell tumors are rare, representing less than 0.1% of all ovarian neoplasms. Among the myriad causes of hirsutism, ovarian tumors account for 1% of the reported cases. We present the case of a 49-year-old parous postmenopausal woman who sought medical attention for hirsutism for 2 years. This case illustrates the unusual and interesting connection between rare ovarian pathology and the clinical manifestation of hirsutism in a postmenopausal patient. Her ultrasonography and MRI showed a right adnexal mass of solid-cystic consistency with thin septations. Her laboratory workup revealed high levels of total testosterone of 256 ng/ml (8.4-48.1ng/ml) and free testosterone of 7.36 pg/ml (0.2-4.1 pg/ml), while DHEAS - 234 µg/dl (35.4-256 µg/dl) and CA125 - 15.8U/L (0.0-35 U/L) were in the normal range. She underwent exploratory laparotomy with a total abdominal hysterectomy and oophorectomy. Histopathological examination and immunohistochemistry conclusively established the presence of a steroid cell tumor, specifically classified as "Not Otherwise Specified"(NOS), in the right ovary.

Effect of Curcumin Gel along with SRP in Chronic Gingivitis Cases

Introduction: Turmeric also known as Curcuma longa thatis widely used as a spice, preservative, coloring agent, and asa household remedy is coming a long way as an alternativemanagement option for periodontal conditions. Curcumin themain yellow bioactive part of turmeric has huge spectrum ofactivity such as anti-inflammatory, antioxidant, antimutagenic,anticarcinogenic, anticoagulant, antidiabetic, antifertility,antibacterial, antiprotozoal, antiviral, antivenom, antiulcer,antifibrotic, hypotensive, and hypocholesteremic activities.The present study was conducted with the aim to determinethe effect of curcumin gel along with SRP in chronic gingivitiscases.Material and methods: The present prospective studyconsisted of 30 subjects as cases and controls respectively.The study was conducted in the department of periodontics ofthe hospital. Scaling was performed by piezoelectric scaler bytrained personnel. After, thorough clearance curcumin gel waslocally applied amongst the cases. All the patients were givensame postoperative instructions and medicaments. Followup was performed after 30 days and the values of gingivalindex were noted. All the data thus obtained was arranged ina tabulated form and analysed using SPSS software. Studentt test was used for statistical analysis and probability value ofless than 0.05 was regarded as significant.Results: The present study consisted of 30 patients as casesand 30 as controls. The mean age of the subjects was 36.42+/-5.22 years. The mean value at day 0 amongst the cases and thecontrols was 2.61+/-0.54 and 2.65+/-0.66 respectively. Themean value at day 30 amongst the cases and the controls was1.90+/-0.38 and 1.45+/-0.45 respectively. On applying studentt test there was a significant improvement amongst the casescompared to the controls on Day 30.Conclusion: Application of curcumin gel could also lead to adecrease in the chances of surgical treatment after SRP as thepocket depth improves significantly

Effect of Curcumin Gel along with SRP in Chronic Gingivitis Cases

Introduction: Turmeric also known as Curcuma longa thatis widely used as a spice, preservative, coloring agent, and asa household remedy is coming a long way as an alternativemanagement option for periodontal conditions. Curcumin themain yellow bioactive part of turmeric has huge spectrum ofactivity such as anti-inflammatory, antioxidant, antimutagenic,anticarcinogenic, anticoagulant, antidiabetic, antifertility,antibacterial, antiprotozoal, antiviral, antivenom, antiulcer,antifibrotic, hypotensive, and hypocholesteremic activities.The present study was conducted with the aim to determinethe effect of curcumin gel along with SRP in chronic gingivitiscases.Material and methods: The present prospective studyconsisted of 30 subjects as cases and controls respectively.The study was conducted in the department of periodontics ofthe hospital. Scaling was performed by piezoelectric scaler bytrained personnel. After, thorough clearance curcumin gel waslocally applied amongst the cases. All the patients were givensame postoperative instructions and medicaments. Followup was performed after 30 days and the values of gingivalindex were noted. All the data thus obtained was arranged ina tabulated form and analysed using SPSS software. Studentt test was used for statistical analysis and probability value ofless than 0.05 was regarded as significant.Results: The present study consisted of 30 patients as casesand 30 as controls. The mean age of the subjects was 36.42+/-5.22 years. The mean value at day 0 amongst the cases and thecontrols was 2.61+/-0.54 and 2.65+/-0.66 respectively. Themean value at day 30 amongst the cases and the controls was1.90+/-0.38 and 1.45+/-0.45 respectively. On applying studentt test there was a significant improvement amongst the casescompared to the controls on Day 30.Conclusion: Application of curcumin gel could also lead to adecrease in the chances of surgical treatment after SRP as thepocket depth improves significantly

An overview of Novel Drug Delivery Systems for Acne

Acne vulgaris is a type of chronic disease of the skin which is mainly caused by the blockage in the sebaceous gland or having any inflammation in those glands which is together is known as pilosebaceous units. It mainly affects the areas having the highest number of sebaceous follicles; those are the face, the back and upper region of the chest. It is a disease that mainly affects the adolescent age group but can be found in any age group. These are present as inflammatory pustules, papules, cysts and nodules, non-inflammatory closed comedones (whiteheads), ripen comedones (blackheads), or a mixture of lesions. Acne is most commonly seen in almost every human being at some point in their lives. There are 20-25 chances of progression of acne to the severe case which leads to permanent scarring. These complications lead to psychological problems like depression, social isolation, lowered self-esteem, and lowered self-confidence. The aim of treating acne is to prevent severe and long-term complications. The present review focuses on novel drug delivery systems for the treatment of acne. It also includes conventional treatments currently available in the market, its limitation and different strategies to overcome these

Role of Periodontist in Screening Diabetes- A Correlation of Clinical and Biochemical Parameters

The increased prevalence and severity of periodontitis seen in patients with diabetes, especially those with poor metabolic control, has led to the designation of periodontal disease as the “sixth complication of diabetes” and successful periodontal therapy in diabetic patients entails the stabilization of blood glucose to a normal range. Hence, present study evaluates whether the noninvasive method of testing gingival blood glucose, is a reliable tool for screening diabetes in chronic periodontitis patients. Methods: The study sample comprised of 75 patients with probing pocket depth of > 4mm and clinical attachment loss of >3mm. Under aseptic measures and proper isolation, after gently probing the gingival sulcus the blood was drawn onto the glucometer strip and the readings were recorded. At the same visit blood was also collected from the index finger onto the glucometer strip. The statistical methods applied were Student's t-test (unpaired) to compare mean values between the two groups and Pearson's correlation for Means and Standard Deviation of the different parameters were calculated. Results: There is a positive correlation between GCBG and CFBG with all the clinical parameters. Fasting blood glucose level is negatively associated with age, plaque index and clinical attachment level (r=0.083, r=0.22 and p=0.042, respectively) whereas Post prandial blood glucose level is negatively associated with age, plaque index and probing pocket depth (r=0.117, r=0.099 and r=0.06, respectively). CFBG is slightly positively correlated with age, plaque index and clinical attachment whereas GCBG is strongly associated with probing pocket depth. Conclusion: The gingival crevicular blood glucose levels also found a positive correlation with the fasting blood glucose levels and post prandial blood glucose levels, suggesting the use of gingival crevicular blood as a screening marker for diabetes. Also a correlation was found between the gingival crevicular blood glucose levels and the clinical parameters suggesting that the treatment of periodontal disease can be considered as an important factor, which can help in improvement of the blood glucose levels in periodontitis subjects.

An Installation Of Apheresis Kit Priming Procedure Had Met With An Accident And Managed Properly.

I would like to illustrate the observational case report of therapeutic plasma phresis kit installation and priming of kit had met a fall out as consequence of leaked of normal saline. We would like to get notified, regarding an event of priming of therapeutic kits their consequence and best management .This incidence was only managed because of keen observation and following of equipment manual instruction. This information has nowhere documented in any trouble shoot management procedure

Behcet’s Syndrome Associated Manifestations and its Treatment Response: A Case Report.

Behçet’s syndrome (BS) or Behcet’s disease (BD) is a chronic multisystem disorder of unknown etiology that is characterized mainly by recurrent mucocutaneous, articular, neurological, gastrointestinal, and ophthalmological lesions. A 20-year-old unmarried, Hindu, male patient presented with a history of recurrent oral and genital ulcers, large joint infl ammatory oligoarthritis predominantly in right knee joint, folliculitis (dermatographia) on the legs, epididymitis, acneiform lesions over face, and panuveitis with associated constitutional symptoms. His Laboratory examinations were nonrevealing except for a moderate rise in erythrocyte sedimentation rate and C-reactive protein. Th is patient meets, in 1990, the International Study Group criteria for BD/BS. Th e main clinical manifestations of this disease are discussed and its early recognition and treatment response is emphasized.

Familial Hypomagnesemia with Secondary Hypocalcemia Mimicking Neurodegenerative Disorder.

Familial hypomagnesemia with secondary hypocalcemia is a genetic disorder of magnesium metabolism that presents with refractory seizures in infancy. Case characteristics: We herein report an infant with familial hypomagnesemia who presented as medically-refractory seizures and had cerebral atrophy on neuroimaging. Interestingly he had lost previous two siblings because of lack of correct diagnosis. Intervention: Child was given oral magnesium supplementation and the seizures got controlled. Message: Familial hypomagnesemia should be considered in any child with recurrent or refractory hypocalcemic seizures.

Correlation of JAK2V617F mutational status in primary myelofibrosis with clinico-hematologic characteristics and international prognostic scoring system scoring: A single center experience.

Introduction: Somatic mutation in the exon 14 of Janus Kinase 2 gene is an established diagnostic marker in bcr-abl negative myeloproliferative neoplasms, especially primary idiopathic myelofi brosis (PIMF). Aim: Our primary aim was to fi nd out the correlation between the JAK2V617F mutational status and the clinico-hematologic characteristics, as well as the international prognostic scoring system (IPSS) scoring of patients with PIMF. Materials and Methods: Clinical and hematologic features were reviewed for 68 patients with primary idiopathic myelofi brosis (PIMF). JAK2V617F mutation status was analyzed by amplifi cation refractory mutation screening-polymerase chain reaction. The patients were further stratifi ed into low, intermediate-1, intermediate-2 and high-risk groups on the basis of IPSS scoring. Results: The JAK2V617F mutation was detected in 58.8% patients. Univariate analysis of variables at presentation identifi ed that JAK2V617F negative patients were signifi cantly associated with more severe anemia (P = 0.045), younger age (P = 0.008), higher transfusion requirement (P = 0.017), and thrombocytopenia (P = 0.015). Patients who were homozygous for JAK2V617F mutation were associated with thrombocytosis (P = 0.014) and also had higher median total leucocyte count (P = 0.20) than the other groups. No signifi cant correlation was detected between JAK2V617F mutational status and the presence of constitutional symptoms, spleen size, grade of bone marrow fi brosis or prognostic risk stratifi cation of the PIMF patients. Conclusion: The variations in the prognostic implication of PIMF patients with mutation status as stated by various publications worldwide, reinstates the need for larger prospective studies using standardized JAK2V617F quantifi cation methods as well as estimation of other newer molecular markers to develop deeper insight into various molecular alterations involving PIMF patients in India as well as worldwide.

Customized Alar Stent Fabrication for Atrophic Rhinitis Patient: A Non-invasive Technique.

Atrophic Rhinitis is a form of chronic rhinitis in which the nasal mucosa atrophies and hardens; eventually causing the nasal passages to dilate and dry out. Other prominent findings include foul smelling crusts and bleeding. Despite various local, medical and surgical methods suggested for the treatment of this slowly progressing disease, the problem with respect to the proper maintenance of the nasal valve area remains unsolved. The alar stent prevents the narrowing of the nostrils and helps in maintaining the airway patency. This article describes a simplified, non-invasive technique for the fabrication of a heat polymerized clear acrylic resin alar stent for treating the atrophic rhinitis patient.

Influence of Abiotic Factors and Infection of Fasciola gigantica on Oviposition of Vector Snail Lymnaea acuminata.

Aims: The aim of the present study is establish a correlation between the abiotic factors and infection rate of Fasciola gigantica and their effect on the reproduction of vector snail Lymnaea acuminata. Place and Duration of Study: Malacology Laboratory, Department of Zoology, DDU Gorakhpur University, Gorakhpur in between November 2009 - October 2010. Methodology: Fecundity of infected/uninfected snail Lymnaea acuminata was noted in different months of year 2009-2010.Fecundity was dependent on variant abiotic environmental factors and infection rate of Fasciola gigantica larvae in snail body. In infected snail the highest fecundity was observed in winter and lowest in summer. In order to ascertain that such a relationship between fecundity and abiotic factors is not coincident, the nervous tissue of the snail was assayed for the activity of the acetylcholinesterase (AChE) in uninfected and infected snails in each months of the year. It was noted that abiotic factors and infection parameter in snail body influence the influence the fecundity of the snails in each month of year 2009-2010. Results: There was a significant positive rank correlation between total fecundity of uninfected/infected snails and corresponding AChE activity in the nervous tissue of same snails.

In vitro assessment of antimicrobial activity of the selected essential oils.

The present study evaluates the antimicrobial activity of three essential oils of Eucalyptus globulus, Eugenia caryophyllata and Psoralea corylifolia. The agar well diffusion method was used to assess the antimicrobial activity against five selected microorganisms S. aureus and B. subtilis (Gram-positive bacteria); E. coli and P. aeruginosa (Gram-negative bacteria) and P. chrysogenum (Fungus). The results revealed that the E. globulus was found to be most significant in comparison to the other oils. All the three oils have revealed higher antimicrobial activity against S. aureus.

Identification of molecular messenger genes in labeo rohita responsible for detection, co-ordination and regulation of its immune system.

Labeo rohita (Rohu) is one of the most important fish with high potential for aquaculture. It is a major constituent of human diet. As the aqua-culturist goes for intensive or semi-intensive culture systems the incidence of disease shall exceed accordingly. To evaluate the impact of stress, it is important to study the molecular messengers in detail. Acquired immunity is not functional in fishes at the stage of hatching. Consequently, at the long run of life, these fishes are dependant on innate immunity for defence against the myriad of microorganisms found in the aquatic habitat. This work was carried out to understand the protective mechanism of rohu against these pathogens. The presence or absence of the molecular messengers found in higher vertebrates, which play a vital role in immune response and regulation was ascertained. Amplification of the genes through RT-PCR technology with novel primers indicated the presence of MHC2b, interleukin 1 beta chain, MHC 2a, MHC 1a, tumour necrosis factor (TNF) and macrophage colony stimulating factor (MCSF) gene. Whereas the presence of Natural Killer cell Enhancing Factor (NKEF), Inducible Nitrous Oxide Synthetase (INOS), Interferon (INF), and β-actin genes could not be justified in this work as no amplification was observed in the RT-PCR experiment.

Antimicrobial, antioxidant and chemopreventive potential of Vitamin C rich fruits.

The aqueous and methanolic extracts of Carambola, Guava. Kiwi, Papaya and Strawberry were evaluated for their antimicrobial, antioxidant and chemopreventive potential. The in vitro antimicrobial activity was performed by Agar well diffusion method Muller Hinton Agar medium against E. coli. and all the extracts showed significant antimicrobial activity. MIC values against E. coli. were also calculated by macrobroth dilution assay. The activity may be due to the high phenolic content in the fruits. Antioxidant capacity and chemopreventive ability of these extracts was also assayed in which Kiwi fruit was best among all the selected fruits. Natural compounds from these fruits can be further extracted and used as an alternative to synthetic drugs.